Non - structural Proteins 7 and 8 of Feline Coronavirus Form 1 a 2 : 1 Heterotrimer that Exhibits Primer - independent RNA 2 Polymerase Activity
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چکیده
Non-structural Proteins 7 and 8 of Feline Coronavirus Form 1 a 2:1 Heterotrimer that Exhibits Primer-independent RNA 2 Polymerase Activity 3 4 Yibei Xiao†, Qingjun Ma†, Tobias Restle, Weifeng Shang, Dmitri I. 5 Svergun, Rajesh Ponnusamy, Georg Sczakiel, Rolf Hilgenfeld* 6 7 Institute of Biochemistry, Institute of Molecular Medicine, Center for 8 Structural and Cell Biology in Medicine, University of Lübeck, 9 Ratzeburger Allee 160, 23538 Lübeck, Germany 10 Graduate School for Computing in Medicine and Life Sciences, 11 University of Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany 12 EMBL Hamburg Outstation, c/o DESY, Building 25a, Notkestr. 85, 22603 13 Hamburg, Germany 14 Laboratory for Structural Biology of Infection and Inflammation, c/o 15 DESY, Building 22a, Notkestr. 85, 22603 Hamburg, Germany 16 Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 17 555 Zu Chong Zhi Rd., Shanghai 201203, China 18
منابع مشابه
Nonstructural proteins 7 and 8 of feline coronavirus form a 2:1 heterotrimer that exhibits primer-independent RNA polymerase activity.
Nonstructural proteins 7 and 8 of severe acute respiratory syndrome coronavirus (SARS-CoV) have previously been shown by X-ray crystallography to form an 8:8 hexadecamer. In addition, it has been demonstrated that N-terminally His(6)-tagged SARS-CoV Nsp8 is a primase able to synthesize RNA oligonucleotides with a length of up to 6 nucleotides. We present here the 2.6-Å crystal structure of the ...
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